Selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. There were a total of 18 shigella isolates and of these, 9 were recovered from direct culture only on hektoen agar compared to 6 from subculture onto xld agar only following enrichment in selenite f broth and 3 were isolated on both direct and subculture. Full text views reflects the number of pdf downloads, pdfs sent to. Remel and oxoid products are now part of the thermo scientific brand, combining powerful manual, semiautomated and fully automated test. Recovery of salmonella by using selenite brilliant green. Section 1 identification of the substancemixture and of the company 1. This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements. The efficiency of strontium selenite and selenite f broths in the isolation of s.
Section 1 identification of the substancemixture and of the. V summary and explanation selenitef broth was devised by leifson,1 who demonstrated that selenite was inhibitory for coliforms and certain other microbial. Selenite broth is inhibitory and recommended for selective isolation of salmonella species. Selenitef broth is an enrichment medium for the isolation of salmonella and. Selenite f broth eb02e typical formula grams per litre peptone 5. Add aseptically 10 g of the food product after thorough mixing of the sample to 100 ml of selenite broth and add a further 10 g to 100 ml of tetrathionate broth.
Composition ingredients gms litre part a peptone 5. Selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. English description selenite cystine broth is a medium used for the selective enrichment of salmonella spp from food, dairy products, water and other materials of sanitary importance. Selenite broth is an enrichment medium used for the selective isolation of. The selective effect is not complete, for although the unwanted species are inhibited during the first 12 hours of incubation, they later. White to light yellow homogeneous free flowing powder part b. This dissertation is brought to you for free and open access by the iowa state university capstones. Bacteriological analytical manual 6th edition 1989 aoac.
Selenite broth selenite f broth twin pack himedia laboratories. It is a buffered lactose peptone broth to which sodium biselenite is added as the selective agent. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that. The decreasing rates were, however, different between thetwoenrichment mediausedfig. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Pi7283 rev 03, 012904 selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species. Selenite f broth principle, uses, preparation and result.
Selenite broth selenite f broth twin pack m052 intended use. An uninoculated tube of the selenitef broth is included in the subculturing and. Of these, 96 76% were isolated with selenite f broth and 118 94%. Not all products are available for sale in all territories. Selenite f broth twin pack m025s recommended is used as an enrichment medium for isolation of shigella from food samples.
Selenite f broth selenite f broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in fecal specimens, and thus, was beneficial in the recovery of. Selenite broth is used as a selective medium for the isolation of salmonella species. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Aug 15, 2019 selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. White to cream crystalline powder colour and clarity of prepared medium. Comparisons showed that the mannitol selenite broth was superior to three other liquid media in its selective value for salmonella typhi and that it was as good as. Of these, 96 76% were isolated with selenite f broth and. Selenite clearing ritual to clear out the old to make way. Selenite broth selenitef broth is used as an enrichment medium. Strontium chloride and strontium selenite enrichment broth. It is important that the sample of broth is taken from just above the faecal debris, ensuring that no debris is taken with the sample. Mccance, in laboratory methods in microbiology, 1966. Determination of selenite uptake in relation to cell mass, 33.
The inhibitory action of selenite on escherichia coli, proteus vulgaris. Recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Original article evaluation of selenite f broth as an. Lello for preparing the samples of strontium selenite used in the investigation and for allowing us to publish the notes on his method of preparing the. Selenite f broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in. Mannitol selenite broth for in vitro use only catalogue no. English description selenite broth is an enrichment medium used for the selective isolation of salmonella and some species of shigella.
Selenitef broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in. Further study of strontium selenite and selenite f broths for. Of these, 96 76% were isolated with selenite f broth and 118 94% were isolated with strontium selenite broth. Levine stated that reduction was intimately associated with vigor of growth, and was caused by the. Reduce media preparation time and simultaneously enrich and isolate salmonella spp.
Criterion selenite cystine broth powder should appear homogeneous, free flowing, and offwhite in color. In the same way that the moon affects drive and intuition, selenite is said to have a similar influencing quality. All lot numbers of selenite broth w and wo cystine have been tested using the following quality control organisms and have been found to be acceptable. In thecaseof selenite f broth the number of positive isolates decreased from 64 to 18 after being kept at room temperaturefor three days, with a reduction. Selenite broth is a selective medium which is inhibitory to organisms such as e. Selenite broth msds search our sds online database free. Sterilise in a boiling water bath, or in free flowing steam, for 10 minutes. Selenite cystine broth liquid medium for detection of salmonella spp according to iso 6785. Sterilize in boiling water bath or free flowing steam for 10 minutes. Selenite cystine broth is a medium used for the selective enrichment of salmonella spp from food, dairy products, water and other materials of sanitary importance. Selenitef broth selenitef broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in fecal specimens, and thus, was beneficial in the recovery of salmonella species. To investigate whether enrichment of clinical faecal specimens in selenite f broth, for the cultivation of salmonella and shigella, should be. Product summary and explanation the growth and isolation of salmonella in food and other samples can be suppressed from foodprocessing procedures including exposure to low and or high temperatures, drying, radiation, preservatives, and. This medium is prepared according to the original formula described as selenite f broth by leifson and.
Selenite cystine broth search our sds online database free. Leifson, in 1936, described the ability of selenite broth to enrich the cultivation of salmonellas while inhibiting other microorganisms. Liefson 2 suggested that it is best to tube the medium to a depth of 2 inches 50mm or more. Selenite broth conforms with the american public health association apha,5 and is specified for clinical applications. Composition ingredients gms litre part a tryptone 5. Selenite broth exist, including selenite cystine broth, from the original formula described as selenite f broth by leifson. If possible even larger samples are preferable with proportionately larger amounts of the enrichment broths being used. A medium for the selective enrichment of salmonella spp from both clinical and food samples. The word selenite comes from the greek word selene, which means moon.
Selenite cystine broth base is modified from the formula of leifson 2 with added cystine 3. Selenite broth liquid medium for selective enrichment of salmonella spp, from clinical and nonclinical samples, according to apha. Selenite cystine broth intended use selenite cystine broth is used as a selective enrichment medium for the isolation of salmonella from feces, foods, water and other materials of sanitary importance. Selenite f broth composition, principle, uses, preparation and result interpretation. Get a printable copy pdf file of the complete article 340k, or click on a page image below to. Strontium selenite was found to be superior to selenite f in the recovery of s. The inhibitory action of selenite on escherichia coli. Selenite broth base contains lactose as a source of fermentable carbohydrate which helps to prevent any rise in ph value during incubation, thereby preventing any loss of selective activity of selenite. N indicates a polymeric substance containing no free radical initiator in its inventory name but is considered to cover the designated polymer made with any free radical initiator regardless of the amount used.
Strontium chloride and strontium selenite enrichment broth media in the isolation of salmonella volume 67 issue 3 j. Section 1 identification of the substancemixture and of. Recovery of salmonella by using selenite brilliant green sulfa enrichment broth. Recovery of salmonella by using selenite brilliant. Shop online for a wide selection of bd bbl mycoflask selenitef broth prepared media for isolation of salmonella from feces, urine, water, foods and other materials of sanitary. The prepared media should appear clear to slightly opalescent, and very light amber in color. Selenite broth selenitef broth intended use selenite broth selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Selenite f broth twin pack mm052 selenite f broth is recommended as an enrichment medium for the isolation of salmonella species from faeces, urine or other pathological materials in accordance with indian pharmacopoeia. Using the selenite clearing energy is helpful for pinpointing mental strife, and taking action against the anxiety. This medium is prepared according to the original formula described as selenite f broth by. Remove the need for pipetting, dispensing or autoclaving with convenient, prefilled and readytouse tubes of selenite broth. Subcultures should be made from the top of the broth after not more than 24 hours incubation as after this time there is. Selenite broth was originated by leifson, while observing good recovery of salmonella spp. Selenite f broth principle, uses, preparation and result interpretation.
Selenite broth is used as an enrichment medium for the isolation of salmonella and shigella from stool, urine, water and food products. The two most frequently used enrichment broths are gramnegative gn broth and selenite f sf broth. Introduction salmonella and shigella are members of the enterobacteriaceae family and are nonlactose fermenting nlf gramnegative bacilli causing. It is recommended by bis committee composition ingredients gms litre part a peptone 5.
Selenite broth is an enrichment medium used for the selective isolation of salmonella and some species of shigella. Further study of strontium selenite and selenite f broths. If these broths are used, the subculture should be performed within 4 to 6 h for gn broth and within 8 to 12 h for sf broth. Criterion selenite cystine broth for the detection of. Page 1 of 7 sds 7155 rev 0 june 2016 section 1 identification of the substancemixture and of the company 1. It is the most widely used enrichment medium to isolate salmonella typhi from fecal specimen of suspected patients andor from carriers during salmonella outbreaks. Leifson devised the original selenite formulation and demonstrated the efficacy of the medium for isolating salmonella from clinical specimens. Sodium selenite has been utilized in studies of cell proliferation and cancer research. Selenite cystine broth for microbiology sigmaaldrich. Microxpress material safety data sheet according to 200158ec doc. Evaluation of selenite f broth as an enrichment step for the isolation. An examination of 1,588 clinical fecal specimens found salmonella in 296 specimens, including 89 cases detected by the directplating xyloselysinedesoxycholate. Bm0360 selenite f broth a medium for the selective enrichment of salmonella spp from both clinical and food samples. Pdf rfactor transfer in selenite and tetrathionate broths.
Selenite f broth culture media online microbiology notes. Leifson found coliforms and fecal streptococci to be inhibited by selenite, thereby permitting growth of salmonella organisms. Summary and explanation leifson found that selenite inhibited fecal streptococci and coliforms during the first 812 hours of incubation, thereby. Intended use hardy diagnostics criterion selenite cystine broth is a selective enrichment for isolation of salmonella spp. Wellcolex colour salmonella test and selenitef broth. Selenite broth is used as a selective enrichment for the cultivation of salmonella spp. Msdss008 section 1 product and company information page product name. Tm28 our mannitol selenite broth is used for the selective enrichment of salmonella species from a variety of samples. This addition has given favourable results in many studies 4. It is used for the selective enrichment of salmonella spp. The efficacy and sensitivity of selenite brilliant green sulfa enrichment sbg broth for the isolation of salmonella from fecal specimens were evaluated by using both clinical and artificially infected artificial fecal specimens.
This medium complies with the recommendations of iso 6785, apha and fda, for the isolation of salmonella. F indicates a substance that is the subject of a section 5f rule under tsca. Thus, when peptone water containing sodium selenite was inoculated with a mixture of selenite resistant and selenite sensitive organisms, the former grew. Shop online for a wide selection of bd bbl mycoflask selenite f broth prepared media for isolation of salmonella from feces, urine, water, foods and other materials of sanitary. Sterilize in a boiling water bath or free flowing steam for 10 minutes. Use selenite broth base for enrichment and isolation of salmonella from feces, urine, water, foods, and materials of sanitary importance.
773 210 153 1032 845 912 1335 1296 500 180 544 128 211 197 1051 750 1397 722 481 1 162 1344 118 1041 1109 1182 723 1495 1039 763 100 1485 1042 328 954 1412 16 151 392 1219 59